bisBenzimide H 33342 trihydrochloride

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	OVCAR-3, A2780/CP70 and IOSE-364 cells were seeded in 24-well plates at 1 × 105 cells/well and incubated overnight. Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. After treatment, cells were stained with 10 μg/mL Hoechst 33342 (Sigma, St. Louis, MO) in PBS for 10 min in the dark at 37 °C. Cell apoptosis was examined under a fluorescence microscope (ZEISS), and data were collected from three independent experiments.

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OVCAR-3, A2780/CP70 and IOSE-364 cells were seeded in 24-well plates at 1 × 105 cells/well and incubated overnight. Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. After treatment, cells were stained with 10 μg/mL Hoechst 33342 (Sigma, St. Louis, MO) in PBS for 10 min in the dark at 37 °C. Cell apoptosis was examined under a fluorescence microscope (ZEISS), and data were collected from three independent experiments.

Publication protocol

Apoptosis assessment by Hoechst 33342 staining

OVCAR-3, A2780/CP70 and IOSE-364 cells were seeded in 24-well plates at 1 × 105 cells/well and incubated overnight. Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. After treatment, cells were stained with 10 μg/mL Hoechst 33342 (Sigma, St. Louis, MO) in PBS for 10 min in the dark at 37 °C. Cell apoptosis was examined under a fluorescence microscope (ZEISS), and data were collected from three independent experiments.

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