Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit purifies RNA from up to 35 mg tissue	- Do not extend the 15 min incubation at 80°C more than 2 min

Upstream tips
- This kit purifies RNA from up to 35 mg tissue
Protocol tips
- Do not extend the 15 min incubation at 80°C more than 2 min

Publication protocol

In a first step, seven RNA extraction kits (Table 1) were tested on rat renal tissue. 12 samples were analyzed for each kit: Four samples with one rat whole kidney section, four samples with two whole kidney sections and four samples with 80–101 LCM glomerular cross-sections. All sections had a thickness of 5 lm. Later, the four kits, which yielded the highest amount of RNA extracted from rat whole kidney sections were used to extract RNA from human renal tissue: Six samples were analyzed for each kit: Four samples with two human kidney biopsy sections and two samples with 80–160 LCM glomerular cross-sections. Human samples had a thickness of 10 lm. Each of the seven different kits was performed according to the manufacturer’s instructions under RNAse-free conditions. The volume of eluate was set to 25 lL in the case of the rat tissues and 15 lL in the case of the human tissue.


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Manufacturer protocol

Download the product protocol from Fisher Scientific for Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE below.

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