miRCURY RNA Isolation Kit

RNA isolation / purification Cells - immortalized A549

Experiment
RNA isolation / purification Cells - immortalized A549
Product
miRCURY RNA Isolation Kit from Exiqon
Manufacturer
Exiqon

Protocol tips

Protocol tips
RNA isolation was performed as recommended by the manufacturers. MS2 RNA (Roche Diagnostics, Vilvoorde, Belgium) was added as a carrier for RNA extraction from plasma, biofluid-derived exosomes, and, when indicated, from A549 cells.

Publication protocol

Total RNA was isolated from 100, 1000, 10 × 103, 25 × 103 (25c), 200 × 103 (200c) and 800 × 103 (800c) cells (the last three conditions were sometimes referred to as low, medium and high cell numbers/densities, respectively), from 50 μl, 100 μl and 200 μl of plasma, and from biofluid-derived exosomes, using Trizol® LS reagent (Life Technologies, Gent, Belgium), miRNeasy® Mini kit (Qiagen, Venlo, Netherlands), miRCURY™ RNA Isolation and miRCURY™ biofluids kits (Exiqon, Vedbaek, Denmark) as indicated in the manuscript. RNA isolation was performed as recommended by the manufacturers. MS2 RNA (Roche Diagnostics, Vilvoorde, Belgium) was added as a carrier for RNA extraction from plasma, biofluid-derived exosomes, and, when indicated, from A549 cells.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized A549 using miRCURY RNA Isolation Kit from Exiqon.

Paper title
Assessing cellular and circulating miRNA recovery: the impact of the RNA isolation method and the quantity of input material
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Manufacturer protocol

Download the product protocol from Exiqon for miRCURY RNA Isolation Kit below.

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