miRCURY RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	RNA isolation was performed as recommended by the manufacturers. MS2 RNA (Roche Diagnostics, Vilvoorde, Belgium) was added as a carrier for RNA extraction from plasma, biofluid-derived exosomes, and, when indicated, from A549 cells.

Protocol tips
RNA isolation was performed as recommended by the manufacturers. MS2 RNA (Roche Diagnostics, Vilvoorde, Belgium) was added as a carrier for RNA extraction from plasma, biofluid-derived exosomes, and, when indicated, from A549 cells.

Publication protocol

Total RNA was isolated from 100, 1000, 10 × 103, 25 × 103 (25c), 200 × 103 (200c) and 800 × 103 (800c) cells (the last three conditions were sometimes referred to as low, medium and high cell numbers/densities, respectively), from 50 μl, 100 μl and 200 μl of plasma, and from biofluid-derived exosomes, using Trizol® LS reagent (Life Technologies, Gent, Belgium), miRNeasy® Mini kit (Qiagen, Venlo, Netherlands), miRCURY™ RNA Isolation and miRCURY™ biofluids kits (Exiqon, Vedbaek, Denmark) as indicated in the manuscript. RNA isolation was performed as recommended by the manufacturers. MS2 RNA (Roche Diagnostics, Vilvoorde, Belgium) was added as a carrier for RNA extraction from plasma, biofluid-derived exosomes, and, when indicated, from A549 cells.

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Manufacturer protocol

Download the product protocol from Exiqon for miRCURY RNA Isolation Kit below.

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