single stranded-DNA Apoptosis ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1×10^4 cells/ml were used for the assay. - Treated with 25 µM myricetin for 48 h at 37°C

Upstream tips
- 1×10^4 cells/ml were used for the assay. - Treated with 25 µM myricetin for 48 h at 37°C

Publication protocol

Role of apoptosis in myricetin-induced cytotoxicity in A2780 and OVCAR3 cells was determined using a single stranded-DNA Apoptosis ELISA kit (EMD Millipore, Billerica, MA, USA) (21). Cultured A2780 and OVCAR3 cells (1×104 cells/ml) were treated with 25 µM myricetin for 48 h at 37°C, and induction of apoptosis in untreated (control) and myricetin-treated cells were determined according to the manufacturer's protocol. The extent of apoptosis was determined from the absorbance measured at 405 nm using a microplate reader and Multiskan EX software, v3.4 (Multiskan EX, Lab systems).

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