NucleoSpin® RNA

RNA isolation / purification Cells - immortalized OVCAR-3

Experiment
RNA isolation / purification Cells - immortalized OVCAR-3
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Cells were then washed twice with sterile saline and total RNA was extracted using the NucleoSpin RNAII kit (MACHEREY&NAGEL, Scientifix Pty Ltd, Clayton, Australia). For this cells were lysed by adding lysis buffer directly onto the cells. RNA extraction including DNase treatment was carried out according to the manufacturer’s protocol. RNA was eluted in 60 µl RNase free water and RNA concentration measured using NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Roskilde, Denmark).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized OVCAR-3 using NucleoSpin® RNA from Macherey Nagel.

Paper title
Careful Selection of Reference Genes Is Required for Reliable Performance of RT-qPCR in Human Normal and Cancer Cell Lines
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

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Videos

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