NucleoSpin® RNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Aliquot rDNase and store at -20 °C.	- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Cells were then washed twice with sterile saline and total RNA was extracted using the NucleoSpin RNAII kit (MACHEREY&NAGEL, Scientifix Pty Ltd, Clayton, Australia). For this cells were lysed by adding lysis buffer directly onto the cells. RNA extraction including DNase treatment was carried out according to the manufacturer’s protocol. RNA was eluted in 60 µl RNase free water and RNA concentration measured using NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Roskilde, Denmark).

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

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