Total RNA Purification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed		Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.

Upstream tips
For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed
Downstream tips
Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.

Publication protocol

Microbial cells were grown in 5 ml of TSB with and without solithromycin at the indicated concentrations. Total RNA was isolated from S. aureus and S. pneumoniae by phenol and CHCl3 extraction (15). RNA was isolated from H. influenzae cells using the total RNA isolation kit from Norgen (Thorold, Canada). RNA quality was examined using an Agilent Bioanalyzer 2100 and the RNA 6000 lab on a chip; 0.5 to 1 μg of total RNA was loaded onto each well of the RNA 6000 chip. Sample preparation and analysis were carried out according to manufacturer's recommendations for total RNA analysis.

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Manufacturer protocol

Download the product protocol from Norgen Biotek for Total RNA Purification Kit below.

Download manufacturer protocol

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