Total RNA Purification Kit from Norgen Biotek has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
5 years ago
Author: Paul G. Macon
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae using Total RNA Purification Kit from Norgen Biotek.
Download the product protocol from Norgen Biotek for Total RNA Purification Kit below.
Download manufacturer protocolCheck out videos that might be relevant for performing RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae using Total RNA Purification Kit from Norgen Biotek. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment