SMARTpool: ON-TARGETplus TP63 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate cells for 1–3 days at 37°C.

Protocol tips
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate cells for 1–3 days at 37°C.

Publication protocol

Astrocytes (DIV10–14) were transfected with 20–30 pmol of scrambled or nuclear factor erythroid 2-relatedfactor 2 (Nrf2)-targeting siRNA (Santa Cruz, catalog numbers SC-37007 and SC-156128, respectively) using Nucleofector II (Amaxa) according to the manufacturer’s protocol. Based on separate experiments with GFP expression plasmid pmaxGFP (Lonza), astrocyte transfection efficiency using this approach is estimated at 70–80%.

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Dharmacon for SMARTpool: ON-TARGETplus TP63 siRNA below.

Download manufacturer protocol

Videos

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