Notch 1 siRNA (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 48h at 37°C.	Screen cells for western blot analysis.

Protocol tips
Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 48h at 37°C.
Downstream tips
Screen cells for western blot analysis.

Publication protocol

Notch-1 or FLOT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (Shanghai, China). A2780 cells were transfected with Notch-1 or FLOT1 siRNAs and corresponding control siRNA for 48 h using Lipofectamine 2000 according to the manufacturer’s instructions. At 24 h after Notch-1 or control siRNA transfection, cells were split into 96-well plates and subjected to G418 (1 mg/ml) selection for 2 weeks. The transcriptional silencing Notch-1 protein was screened using western blot assay. All transfection experiments were performed at least three times.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Notch 1 siRNA (h) below.

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