Flotillin-1 siRNA (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate for 5 minutes at room temperature. Incubate cells for 48h at 37°C	Screen using western blot assay

Protocol tips
Incubate for 5 minutes at room temperature. Incubate cells for 48h at 37°C
Downstream tips
Screen using western blot assay

Publication protocol

Notch-1 or FLOT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (Shanghai, China). A2780 cells were transfected with Notch-1 or FLOT1 siRNAs and corresponding control siRNA for 48 h using Lipofectamine 2000 according to the manufacturer’s instructions. At 24 h after Notch-1 or control siRNA transfection, cells were split into 96-well plates and subjected to G418 (1 mg/ ml) selection for 2 weeks. The transcriptional silencing Notch-1 protein was screened using western blot assay. All transfection experiments were performed at least three times.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - A2780 FLOT1 using Flotillin-1 siRNA (h) from Santa Cruz Biotechnology.

Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Flotillin-1 siRNA (h) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - A2780 FLOT1 using Flotillin-1 siRNA (h) from Santa Cruz Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.