Bim siRNA (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 1–3 days at 37°C.	Screen cells for western blot analysis and histone/DNA ELISA

Protocol tips
Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 1–3 days at 37°C.
Downstream tips
Screen cells for western blot analysis and histone/DNA ELISA

Publication protocol

Akt and FOXO3a were purchased from Millipore; Bim and control small interfering RNA (siRNA) were purchased from Santa Cruz Biotechnology Inc. Human ovarian cancer A2780 and A2780/DDP cells were transfected with Akt, FOXO3a, Bim and control siRNA using Lipofectamine™ 2000, according to the manufacturer's instructions (31). The cells were then collected and processed for western blot analysis and histone/DNA ELISA.

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Manufacturer protocol

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