SignalSilence® PTEN siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 48h at 37°C.	Screen cells for western blot analysis.

Protocol tips
Add diluted DNA to diluted Lipofectamine® 2000 Reagent and incubate for 5 minutes at room temperature. Incubate cells for 48h at 37°C.
Downstream tips
Screen cells for western blot analysis.

Publication protocol

A SignalSilence PTEN siRNA kit was purchased from Cell Signaling Technology (CST). A2780 cells were transfected with PTEN siRNA and negative control siRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. The transfection efficiency was evaluated by fluorescence microscopy by calculating the percentage of fluorescein-labelled cells. At 48 h after the transfection of the siPTEN, the cells were then subjected to viability and western blot assays for PTEN, pAkt.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for SignalSilence® PTEN siRNA below.

Download manufacturer protocol

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