ON-TARGETplus Human IRF3 (3661) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-100 nM. Incubate TransIT-siQUEST Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells. Incubate for 24–72 hours. It is not necessary to replace the complete growth medium with fresh medium. After 48h inoculate with RSV.

Protocol tips
siRNA concentration-100 nM. Incubate TransIT-siQUEST Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells. Incubate for 24–72 hours. It is not necessary to replace the complete growth medium with fresh medium. After 48h inoculate with RSV.

Publication protocol

Control, IRF3, and CDK9 siRNAs (Dharmacon, ThermoFisher Scientific, Lafayette, CO) were reverse transfected by plating trypsinized A549 cells into a dish containing 100 nM siRNA-TransIT-siQUEST complexes (Mirus Bio Corp.). Forty-eight hours later, cells were inoculated with RSV (multiplicity of infection [MOI], 1.0) or electroporated with poly(I·C) for indicated times. At indicated periods, cells were washed with phosphate-buffered saline (PBS) twice and lysed in Tri reagent (Sigma-Aldrich).

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human IRF3 (3661) siRNA - SMARTpool below.

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