ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature. Add mixture to cells and incubate for 20 minutes at room temperature. Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 48 hours.

Protocol tips
Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature. Add mixture to cells and incubate for 20 minutes at room temperature. Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 48 hours.

Publication protocol

Emmprin, LYVE-1, BCRP and non-target small interfering RNAs were purchased from the manufacturer (ON-TARGET plus SMART pool, Dharmacon, Lafayette, CO, USA). Cells were incubated with small interfering RNAs in 12-well plates using DharmaFECT Transfection Reagent (Dharmacon) according to the manufacturer’s instructions, and gene silencing assessed using immunoblots within 48 h.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool below.

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