Nox3 siRNA (h)

siRNA / miRNA gene silencing Human - BEAS-2B NOX3

Experiment
siRNA / miRNA gene silencing Human - BEAS-2B NOX3
Product
Nox3 siRNA (h) from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
siRNA concentration-50nM

Add diluted siRNA to diluted Lipofectamine Reagent
Incubate for 5 min at RT and add to cells.
Incubate cells for 2 days at 37°C.

Publication protocol

DNA and siRNA transfections were performed using Lipofectamine 2000 according to the manufacturer's recommendations. For general silencing experiments, nonsilencing siRNA (50nM) or gene-specific siRNA (50nM) was transfected into Beas-2B. The nonsilencing siRNA was used as a negative control. After 48 h transfection, the cells were starved for 16 h followed by treatment with or without Cr(VI). For generation of stable cell lines, after transfection of SOD1, SOD2, CAT, shRNA-p47phox, or their responding vectors using Lipofectamine 2000, cells were cultured in complete medium in the presence of 1000 μg/ml G418. After 3 weeks, cell clones resistant to G418 were isolated. Transfected cells were routinely cultured in the presence of 800 μg/ml of G418. Overexpression of SOD1, SOD2, CAT, and suppressed p47phox protein production were confirmed by immunoblotting as described previously (Wang et al., 2010).

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Papers

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Paper title
NADPH Oxidase Activation Is Required in Reactive Oxygen Species Generation and Cell Transformation Induced by Hexavalent Chromium
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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for Nox3 siRNA (h) below.

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