siRNA FOXM1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 1.5–6 x 10^5 cells/weel in a 6 well plate.	siRNA final concentration - 50 nM Incuabte cells for 24h and add diluted siRNA to HiPerFect Transfection Reagent to make a mixture Incubate the mixture for 5–10 min at room temperature. Add mixture to cells and incubate for 72 h

Upstream tips
Seed 1.5–6 x 10^5 cells/weel in a 6 well plate.
Protocol tips
siRNA final concentration - 50 nM Incuabte cells for 24h and add diluted siRNA to HiPerFect Transfection Reagent to make a mixture Incubate the mixture for 5–10 min at room temperature. Add mixture to cells and incubate for 72 h

Publication protocol

Two different FOXM1 small interfering RNAs (siRNAs) and non-silencing control siRNAs were purchased from Sigma-Aldrich. Exponentially growing untreated cells were plated 24 h before transfection. Plated cells were transfected with FOXM1 siRNA or a control siRNA at a final concentration of 50 nM for 72 h, using HiPerFect Transfection Reagent (Qiagen, Valencia, CA) according to the manufacturer's protocol. The concentrations of siRNAs were chosen based on dose-response studies. Non-silencing control siRNA-transfected cells were used as negative controls. After treatment, the cells were harvested and processed for further analysis.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for siRNA FOXM1 below.

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