siGENOME Human RAB5A (5868) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration is 1 nM. Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization. Remove culture medium from cells and add incubate mixture. Incubate the plate at 37°C for 24 to 72 h.

Protocol tips
siRNA concentration is 1 nM. Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization. Remove culture medium from cells and add incubate mixture. Incubate the plate at 37°C for 24 to 72 h.

Publication protocol

Non-targeted siRNA sequence: sense, 5′-UUCUCC GAACGUGUCA CGUdTdT-3′; antisense, 5′-ACGGUG ACACGUUCGGAGAAdTdT-3′, c-Cbl siRNA sequences: sense, 5′-GGGAAGGCUUCUAUUUGUUdTdT-3′; antisense, 5′-AACAAAUAG AAGCCUUCCCdTdT-3′, p62 siRNA sequence: sense, 5′-GCAUUGAAGUUGAUAUCGAUd TdT-3′; antisense, 5′-AUCGAUAUCAACUUCAAUGC dTdT-3′, NBR1 siRNA sequence: sense, 5′-GGAGUGG AUUUACCAGUUAUUdTdT-3′;antisense, 5′-AAUAA CUGGUAAAUCCACUCCdand FAK siRNA sequence: sense, 5′-GCGAUUAUAUGUUAGAGAUAG dTdT-3′; antisense, 5′-CUAUCUCUAACAUAUAAUCGCdTdT-3′ were from Dharmacon. Src siRNA was from Sigma with the following sequences: sense, 5′-CUGAGAGGG CGGUGGGUA UdTdT-3′ (WD04736566); anti-sense, 5′-AUACCCACCGCCCUCUCAGdTdT-3′ (WD04736567). siRNA for Rab5a: sequence 1 (siGENOME siRNA D-004009-01); and sequence 2 (siGENOME siRNA D-004009-02); Atg12: sequence 1 (siGENOME siRNA D010212-03); and sequence 2 (siGENOME siRNA D010212-05) were from Thermo Fisher Scientific. Control siRNA (Cell Signalling) or target siRNA oligonucleotides were expressed in cells by incubated with INTERFERin (Polyplus transfection) in serum-free RPMI medium according to the manufacturer's instruction.

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Manufacturer protocol

Download the product protocol from Dharmacon for siGENOME Human RAB5A (5868) siRNA - SMARTpool below.

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