siRNA RIP3

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 3 days at 37°C.

Protocol tips
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 3 days at 37°C.

Publication protocol

Transfection of siRNA was performed using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions. RIP3 siRNA (sc-61482) was purchased from Santa Cruz Biotechnology. The control siRNA (6568) was purchased from Cell Signaling Technology. Three days after siRNA transfection, the cells were used for subsequent experiments.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for siRNA RIP3 below.

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