siRNA Junction plakoglobin (SASI_Hs01_00246520)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 200 000 cells in 6 well plate for 24 hours	siRNA concentration-40nm Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1 day at 37°C and replace medium. Incubate for another 48 hours.

Upstream tips
Seed 200 000 cells in 6 well plate for 24 hours
Protocol tips
siRNA concentration-40nm Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1 day at 37°C and replace medium. Incubate for another 48 hours.

Publication protocol

The day before transfection 200 000 cells were plated out in 6 well plates. Transfection were done with 40 nM of either MISSION siRNA Universal Negative Control #1 with no homology to all mature and predicted RefSeq mRNA sequences (SIC001, Sigma-Aldrich) or MISSION plakoglobin siRNA (ID: SASI_Hs01_00246520, Sigma-Aldrich) using 5 µl of LF2000 in a total of 2.5 ml growth medium without antibiotics. After 24 hours the medium was replaced and the cells were incubated for another 48 hours before analysis/harvesting.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for siRNA Junction plakoglobin (SASI_Hs01_00246520) below.

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