ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-150 nm. Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 2 days at 37°C.

Protocol tips
siRNA concentration-150 nm. Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 2 days at 37°C.

Publication protocol

SMARTpool siRNAs targeting CTHRC1 and DPAGT1 (referred to as S siRNA) were obtained from Dharmacon. The non-silencing negative control siRNA (referred to as NS siRNA) was from Qiagen. CAL27 cells were transfected at 60% confluence with either NS or S siRNA (150 nm) using Lipofectamine 2000 (Invitrogen) and cultured for 48 h. Total RNAs isolated from CAL27 cells transfected with either NS or S siRNA were used for cDNA synthesis to assess CTHRC1 and DPAGT1 expression by real-time PCR. The gene expression profiles were generated by normalizing the Ct (threshold cycle numbers) of CTHRC1 and DPAGT1 with a housekeeping gene (18 S rRNA) and comparing the gene expression of cells treated with NS or S siRNA.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human DPAGT1 (1798) siRNA - SMARTpool below.

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