ON-TARGETplus Human ITGB4 (3691) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-50 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 2 days at 37°C.

Protocol tips
siRNA concentration-50 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 2 days at 37°C.

Publication protocol

For silencing of integrin subunitsβ5orαv, ILK or Src, 50 nM ofthe predesigned integrin-specific siRNA sequences (Silencer SelectPredesigned siRNA; Ambion, USA; siRNA ID# s7591 (si(β5)), s7568(si(αv)), s7404 (si(ILK)), s13414 (si(Src)) and control human nonspe-cific siRNA (si(−)) (Silencer Select Predesigned siRNA Negative Control#1 siRNA; Ambion) were used. The transfection of siRNAs was per-formed using Lipofectamine RNAiMAX Reagent (Invitrogen) accordingto the manufacturer's instructions. Twenty four hours after transfectioncells were plated and 24 h later treated with anticancer drugs for assess-ment of cell survival.

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Manufacturer protocol

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