siRNA ITGAV

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-50 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 2 days at 37°C.

Protocol tips
siRNA concentration-50 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 2 days at 37°C.

Publication protocol

For silencing of integrin subunitsβ5orαv, ILK or Src, 50 nM ofthe predesigned integrin-specific siRNA sequences (Silencer SelectPredesigned siRNA; Ambion, USA; siRNA ID# s7591 (si(β5)), s7568(si(αv)), s7404 (si(ILK)), s13414 (si(Src)) and control human nonspe-cific siRNA (si(−)) (Silencer Select Predesigned siRNA Negative Control#1 siRNA; Ambion) were used. The transfection of siRNAs was per-formed using Lipofectamine RNAiMAX Reagent (Invitrogen) accordingto the manufacturer's instructions. Twenty four hours after transfectioncells were plated and 24 h later treated with anticancer drugs for assess-ment of cell survival.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - CAL-27 ITGAV using siRNA ITGAV from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA ITGAV below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - CAL-27 ITGAV using siRNA ITGAV from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.