Cell Meter™ Autophagy Assay Kit *Green Fluorescence

Autophagy assay cell type - Mesenchymal stromal cells

Experiment
Autophagy assay cell type - Mesenchymal stromal cells
Product
Cell Meter™ Autophagy Assay Kit *Green Fluorescence from AAT Bioquest
Manufacturer
AAT Bioquest

Protocol tips

Protocol tips
Autophagosomes were detected with an autofluorescent compound monodansylcadaverine (MDC), using a Cell Meter Autophagy kit (AAT Bioquest, Inc.). Briefly, BMSCs were seeded on glass coverslips in a 24-wells plate at a density of 3 x 104/well. After incubation, BMSCs were stained with 1 x MDC solution and incubated at 37°C for 1 hour. Cells were washed three times and examined under a confocal microscope (Ex = 330 nm).

Publication protocol

Autophagosomes were detected with an autofluorescent compound monodansylcadaverine (MDC), using a Cell Meter Autophagy kit (AAT Bioquest, Inc.). Briefly, BMSCs were seeded on glass coverslips in a 24-wells plate at a density of 3 x 104/well. After incubation, BMSCs were stained with 1 x MDC solution and incubated at 37°C for 1 hour. Cells were washed three times and examined under a confocal microscope (Ex = 330 nm).

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Manufacturer protocol

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