Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
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siRNA concentration-100 nM
Add 7.5 µl TransIT-X2 to the diluted DNA mixture and incubate at room temperature for 15–30 minutes.
Add the TransIT-X2:DNA complexes to cells and incubate for 48 hours.
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|
Protocol tips |
siRNA concentration-100 nM
Add 7.5 µl TransIT-X2 to the diluted DNA mixture and incubate at room temperature for 15–30 minutes.
Add the TransIT-X2:DNA complexes to cells and incubate for 48 hours.
|
Publication protocol
COV434 and KGN cells were seeded in 6-well plates and cultured until 70% confluency was obtained. TransIT-X2 Dynamic Delivery System was used to perform siRNA transfection at concentrations of 12.5, 25, 50 and 100 nM. The cells were incubated for 48 h post transfection, then harvested to determine the transfection efficiency by JNK expression on western blot.
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Papers
Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - COV-434 SAPK/JNK using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb from Cell Signaling Technology.
Paper title
Endogenous c-Jun N-terminal kinase (JNK) activity marks the boundary between normal and malignant granulosa cells
Manufacturer protocol
Download the product protocol from Cell Signaling Technology for Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb below.
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