Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-100 nM Add 7.5 µl TransIT-X2 to the diluted DNA mixture and incubate at room temperature for 15–30 minutes. Add the TransIT-X2:DNA complexes to cells and incubate for 48 hours.

Protocol tips
siRNA concentration-100 nM Add 7.5 µl TransIT-X2 to the diluted DNA mixture and incubate at room temperature for 15–30 minutes. Add the TransIT-X2:DNA complexes to cells and incubate for 48 hours.

Publication protocol

COV434 and KGN cells were seeded in 6-well plates and cultured until 70% confluency was obtained. TransIT-X2 Dynamic Delivery System was used to perform siRNA transfection at concentrations of 12.5, 25, 50 and 100 nM. The cells were incubated for 48 h post transfection, then harvested to determine the transfection efficiency by JNK expression on western blot.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - COV-434 SAPK/JNK using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb from Cell Signaling Technology.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - COV-434 SAPK/JNK using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.