MouseTRAP™ (TRAcP 5b) ELISA

Protocol tips

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- Cell were grown with α-MEM containing RANKL (40 ng/ml) and the medium is changed and the supernatant is collected every 2 days (3, 5 and 7 days) and stored at -80C

Upstream tips
- Cell were grown with α-MEM containing RANKL (40 ng/ml) and the medium is changed and the supernatant is collected every 2 days (3, 5 and 7 days) and stored at -80C

Publication protocol

RAW 264.7 cells were cultured with α-MEM containing RANKL (40 ng/ml) for 3, 5 and 7 days with a change of medium every two days and the supernatants on days 3, 5 and 7 were collected and stored at −80°C. A MouseTRAP™ enzyme immunoassay (EIA) kit (Immunodiagnostic Systems Ltd., Boldon, UK) was used in the determination of the level of TRACP 5b in the supernatants according to the instructions provided by the manufacturer. The intra-assay variation of the method for TRACP 5b was <6.5% and the inter-assay variation was <8%. The standard sample supplied by the kit was 2.0 (1.6–2.5) U/l, and the obtained result in our laboratory was 1.94 U/l.

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Manufacturer protocol

Download the product protocol from Immunodiagnosticsystems (ids) for MouseTRAP™ (TRAcP 5b) ELISA below.

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