siRNA FOXL2

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 300,000 cells per well of a six well plate.	Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 7h at 37°C and replace medium.

Upstream tips
Seed 300,000 cells per well of a six well plate.
Protocol tips
Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 7h at 37°C and replace medium.

Publication protocol

For siRNA knockdown of mutant FOXL2, KGN cells, passage 8, were seeded at a density of 300,000 cells per well of a six well plate. Cells were transfected with FOXL2 stealth RNAi™ (cat#HSS101080 Invitrogen, NZ) or a GC matched control using Lipofectamine 2000 and Opti-MEM serum free media (Invitrogen, NZ). RNAi-lipid complexes were removed 7 h post transfection and replaced with complete media. For overexpression of wildtype or mutant FOXL2, COV434 cells, passage 9, were seeded at a density of 300,000 cells per well of a six well plate. Cells were transfected with 1 µg of control (empty vector), pFOXL2wt, or pFOXL2m using Lipofectamine 2000 and Opti-MEM serum free media. Plasmid-lipid complexes were removed 7 h post transfection and replaced with complete media. All transfections were performed in triplicate.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA FOXL2 below.

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