CytoTox 96® Non-Radioactive Cytotoxicity Assay

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		- LDH activity was measured after 24 h of incubation

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- LDH activity was measured after 24 h of incubation

Publication protocol

Toxicity was assayed by measuring the activity of the cytosolic enzyme, lactate dehydrogenase (LDH), released into the culture medium after the membrane damage (27). Samples from clarified medium of treated and untreated control wells were taken after 24 h of incubation and the LDH activity was measured using the cytotoxicity assay, CytoTox 96® (Promega, USA) associated with a fully automated microplate reader photometer (BioTek, USA).

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