siRNA PIN1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Pin1 shRNA-1: 5’-CGGCAACAGCAGCAGUGGUGGCAAA-3’ Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C.

Protocol tips
Pin1 shRNA-1: 5’-CGGCAACAGCAGCAGUGGUGGCAAA-3’ Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C.

Publication protocol

For the knockdown of human Pin1, the siRNAs against Pin1 (Pin1 shRNA-1: 5’-CGGCAACAGCAGCAGUGGUGGCAAA-3’ and Pin1 shRNA-2: 5’-GCCCUGGAGCUGAUCAACGGCUACA-3’) and control siRNA were purchased from Invitrogen (Stealth/siRNA duplex oligoribonucleotides), and transfected into LNCaP or DU145 cells using Lipofectamine RNAi Max (Invitrogen, CA), according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA PIN1 below.

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