siRNA SKIL

siRNA / miRNA gene silencing Human - DuCaP SKIL

Experiment
siRNA / miRNA gene silencing Human - DuCaP SKIL
Product
siRNA SKIL from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Seed 30000 cells/well
Protocol tips
The siRNA sequences (5′-to-3′) were:

1: GGCAAGUAAGUCCAUAUCATT (sense) and UGAUAUGGACUUGCCTC (antisense)

2: GGCUCACAGUAGUGGUAATT (sense) and UUACCACUACUGUGAGCCTT (antisense)

SKIL siRNA concentration- 50 nM

Add INTERFERin to siRNA duplexes and incubate for 10min at RT after homogenization.

Remove culture medium from cells and add incubate mixture.

Incubate the plate at 37°C for 24 to 72 h.

Publication protocol

The knockdown of SKIL expression was done using small interfering RNA (AM16708, ID 107695) from Ambion (Ambion, Austin, TX, USA). The siRNA sequences (5′-to-3′) were:

1: GGCAAGUAAGUCCAUAUCATT (sense) and UGAUAUGGACUUGCCTC (antisense)

2: GGCUCACAGUAGUGGUAATT (sense) and UUACCACUACUGUGAGCCTT (antisense)

Silencer® Negative Control #1 siRNA (AM4611, Ambion, Austin, TX, USA) was used as a non-targeting control. Cells were seeded into 24-well plates (30000 cells/well) in four replicates and were transfected the following day with 50 nM SKIL siRNA and scrambled siRNA. INTERFERin TM (PolyPlus Transfection, Strasbourg, France) and Opti-MEM were used for cell transfection.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - DuCaP SKIL using siRNA SKIL from Thermo Fisher Scientific.

Paper title
Recurrent SKIL-activating rearrangements in ETS-negative prostate cancer
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA SKIL below.

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