Thiazolyl Blue Tetrazolium Bromide

Cell cytotoxicity / Proliferation assay cell type - HUVEC

Experiment
Cell cytotoxicity / Proliferation assay cell type - HUVEC
Product
Thiazolyl Blue Tetrazolium Bromide from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 1 × 104 cells/ml cells were platted in a 96 well plate.

Cells were pretreated with different concentrations of resveratrol
Protocol tips
- Cells were treated with 0.5 mg/ml MTT

Publication protocol

Cell proliferation was measured using an MTT assay. Briefly, cells (1 × 104 cells/ml) were seeded in 96-well plates, and after 24 h, treated with different concentrations of resveratrol (0.1∼50 μm). After treatment, media was removed, cells were washed twice with PBS, and 200 μl of a 0.5 mg/ml MTT solution in PBS was added to each well. Plates were incubated at 37 °C for 4 h, MTT solution was removed, and cells were lysed using a solubilization solution (1:1 DMSO:ethanol). The formazan dye product was quantified using an ELISA microplate reader at an absorbance of 560 nm. To examine the protective effect of the AMPK inhibitor (Compound C), cells were pretreated with Compound C 1 h before being treated with resveratrol.

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Manufacturer protocol

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