ON-TARGETplus Human PLK1 (5347) siRNA - Individual

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Plk1-Si1:AACCAGUGGUUCGAGAGACAG Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C.

Protocol tips
Plk1-Si1:AACCAGUGGUUCGAGAGACAG Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1–3 days at 37°C.

Publication protocol

Small interfering RNA sequences. Plk1 siRNAs were purchased from Dharmacon Research, Inc. (Lafayette, CO): Plk1-Si1 (AACCAGUGGUUCGAGAGACAG) and Plk1 Si1C (AACCAGUUUGGCGAGAGACAG). The selection of these sequences was based on the sequence of the best Plk1 AS oligo isis121969 (GAACCAGUGGUUCGAGAGACA) obtained from ISIS Pharmaceuticals (Carlsbad, CA; data not shown).

Transfection. siRNA was transfected using LipofectAMINE 2000 (LF2000) from Invitrogen (Carlsbad, CA) according to the manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human PLK1 (5347) siRNA - Individual below.

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