siRNA CDKN1A

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-30 nM Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1 day at 37°C.

Protocol tips
siRNA concentration-30 nM Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 1 day at 37°C.

Publication protocol

HT-29 cells were cultured as described above. After 2 days of culture, the culture medium was changed to RPMI 5% FCS and small interfering RNA (siRNA) against p21 (Silencer Select ID s415) (30 nM) or negative scramble sequence (Silencer Select Negative Control no. 1) (30 nM) were added together with the lipofectamine RNAiMax Transfection Reagent dissolved in Opti-MEM medium according to the manufacturer’s instructions (all from Life Technologies). Following a 24 h transfection, the transfection media were removed and cells were treated as described in Fig. 1 and effects of p21 siRNA knockdown were analysed with respect to cell count, cell growth recovery, cell cycle distribution, and p21 expression.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA CDKN1A below.

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