Cell Counting Kit-8

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 10 μL CCK-8 reagent and incubate at 37 °C for 1 h.

Protocol tips
Add 10 μL CCK-8 reagent and incubate at 37 °C for 1 h.

Publication protocol

A proliferation assay was carried out using Cell Counting Kit-8 (CCK-8, Dojindo Laboratories, Japan) according to the manufacturer’s protocol [17]. Briefly, 2000 cells/well were seeded into 96-well plates and transfected with indicated siRNA. The cells were cultured for 0, 24, 48 and 72 h, then 10 μL CCK-8 reagent was added to each well and the cells were incubated at 37 °C for 1 h. The absorbance was recorded at 450 nm with a microplate reader (Bio-Rad).

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Manufacturer protocol

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