ON-TARGETplus Human MID1 (4281) siRNA - Individual

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Final concentration of MID1-20 to 40 nM. MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′ Add diluted siRNA to diluted NanoFectin reagent. Incubate for 15 min at RT and add to cells.  Replace medium after 48hand re-incubate cells for 72-120h at 37°C.

Protocol tips
Final concentration of MID1-20 to 40 nM. MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′ Add diluted siRNA to diluted NanoFectin reagent. Incubate for 15 min at RT and add to cells.  Replace medium after 48hand re-incubate cells for 72-120h at 37°C.

Publication protocol

Cells were transfected with 20 to 40 nM final concentration of MID1, α4, AR or control siRNA oligonucleotides using Lipofectamin 2000 or Nanofectin. AR siRNA: 5′-GAAAGCUCCUCGGUAGGUC-3′; α4-4 siRNA: 5′-UUGAGAUGCCAUAGCAACGAG-3′; MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′; ns siRNA: 5′-AAGAGGCUUGCACAGUGCA-3′ (Dharmacon or MWG). After 48 h of incubation the medium was replaced and after 72–120 h cells were harvested and analyzed by Western blotting or real-time PCR.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human MID1 (4281) siRNA - Individual below.

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