MTT Cell Growth Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 3000 cells/well were seeded on a 96 well plate. These cells were pretreated /transfected with miR-143 mimic, or control mimic for 48 h	- Cellls were treated with MTT at 50 mg per well

Upstream tips
- 3000 cells/well were seeded on a 96 well plate. These cells were pretreated /transfected with miR-143 mimic, or control mimic for 48 h
Protocol tips
- Cellls were treated with MTT at 50 mg per well

Publication protocol

The cancer cells were transfected with miR-143 mimic, or control mimic for 48 h. Cells were then seeded in a 96-well plate, at a density of 3000 cells/well for overnight incubation. The cell proliferation rates were measured with MTT assay (Sigma–Aldrich, Inc., St. Louis, MO, U.S.A.). Briefly, cells were treated with MTT at 50 mg per well. The generated formazan was dissolved in DMSO, and the absorbance was recorded by measuring the absorbance at 590 nm with a plate reader. The same experiment was repeated three times.

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