DeadEnd™ Fluorometric TUNEL System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- When there was a little or poor staining, optimise permeabilization step by adjusting incubation time with permeabilization agent. - When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Downstream tips
- When there was a little or poor staining, optimise permeabilization step by adjusting incubation time with permeabilization agent. - When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Publication protocol

We applied MTT assay to determine cell viability. The number of cells was counted every 24 h for 7 days. A TUNEL apoptosis detection kit (Promega) was used for DNA fragmentation fluorescence staining according to the manufacturer’s protocol. Positively stained, fluorescein-labelled cells were visualised and counted using a fluorescence microscope (Nikon Eclipse 80i, China).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using DeadEnd™ Fluorometric TUNEL System from Promega.

Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Fluorometric TUNEL System below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - HeLa cells human cervical cancer using DeadEnd™ Fluorometric TUNEL System from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.