LC3B Antibody

Autophagy assay cell type - Keratinocytes

Experiment
Autophagy assay cell type - Keratinocytes
Product
LC3B Antibody from Novus Biologicals
Manufacturer
Novus Biologicals

Protocol tips

Protocol tips
Dilute primary Ab at 1:400 and incubate overnight

Publication protocol

Immunofluorescence analysis for LC3 was performed, as described previously (18). Briefly, cells were fixed in 4% paraformaldehyde at 4°C for 10 min. They were then permeabilized with 0.01% Triton X-100, before being blocked with 10% BSA for 1 h. Cells were stained with primary Ab (rabbit anti-human LC3, diluted 1:400) overnight, and then incubated with secondary Ab (anti-rabbit IgG-tetramethylrhodamine isothiocyanate or anti-rabbit IgG-Alexa488) for 1 h. Unbound Ab was removed with PBS, and cells were imaged using a Zeiss LSM510 META confocal microscope (Zeiss).

Full paper   Login or join for free to view the full paper.

Reviews

LC3B Antibody from Novus Biologicals has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - Keratinocytes using LC3B Antibody from Novus Biologicals.

Paper title
Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Novus Biologicals for LC3B Antibody below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - Keratinocytes using LC3B Antibody from Novus Biologicals. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms