Accell Human VDAC1 (7416) siRNA - Set of 4

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 150,000 cells/well	siRNA concentration- 5–100 nmol/l si-hVDAC1- S: 238-5′ACACUAGGCACCGAGAUUA3′-256 and AS: 5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 1/A- S: -238-5′ACACUAGGCACCGAGAUUA 3′- 256, and AS: 5′UAAUCUCGGUGCCUAGUGU3′-256; si-hVDAC1 2/A- S: 238-5′ ACACUAGGCACCGAGAUUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 2/B-, S: 238- 5′ACACUAGGCACCGAGA UUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′-256 Dilute siRNA and mix with jetPRIME® reagent. Incubate for 10 to 15 min at RT and add to cells. Incubate cells at 37 °C for 24 hours and change medium

Upstream tips
Seed 150,000 cells/well
Protocol tips
siRNA concentration- 5–100 nmol/l si-hVDAC1- S: 238-5′ACACUAGGCACCGAGAUUA3′-256 and AS: 5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 1/A- S: -238-5′ACACUAGGCACCGAGAUUA 3′- 256, and AS: 5′UAAUCUCGGUGCCUAGUGU3′-256; si-hVDAC1 2/A- S: 238-5′ ACACUAGGCACCGAGAUUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 2/B-, S: 238- 5′ACACUAGGCACCGAGA UUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′-256 Dilute siRNA and mix with jetPRIME® reagent. Incubate for 10 to 15 min at RT and add to cells. Incubate cells at 37 °C for 24 hours and change medium

Publication protocol

Nonmodified and 2′-O-methyl-modified hVDAC1-siRNAs were synthesized by Dharmacon or were obtained from Genepharma (Suzhou, China). The following sequences (names underlined) were used, with 2′-O-methyl-modified nucleotides indicated in bold and underlined (nucleotide positions are provided for sense (S) and antisense (AS) sequences): si-hVDAC1- S: 238-5′ACACUAGGCACCGAGAUUA3′-256 and AS: 5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 1/A- S: -238-5′ACACUAGGCACCGAGAUUA 3′- 256, and AS: 5′UAAUCUCGGUGCCUAGUGU3′-256; si-hVDAC1 2/A- S: 238-5′ ACACUAGGCACCGAGAUUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′; si-hVDAC1 2/B-, S: 238- 5′ACACUAGGCACCGAGA UUA3′-256 and AS: 238-5′UAAUCUCGGUGCCUAGUGU3′-256; si-mVDAC1- S: 238-5′ACCCUGGGCACUGAGAUCA3′-256 and AS: 238-5′UGAUCUCAGUGCCCAGGGU 3′-256; and Scrambled- S: -5′GCAAACAUCCCAGAGGUAU3′ and AS : -5′AUACCUCUGGGAUGUUUGC3′.

Cells were seeded (150,000 cells/well) on six-well culture dishes to 30–50% confluence and transfected with 5–100 nmol/l siRNA-hVDAC1 using the JetPRIME reagent in the case of HeLa and A549, PC-3, HepG2, U87, HaCat, and Min-6 and Panc-1cells, while HCT116 and HEK293, cells were transfected using Dharmafect, all according to the manufacturers' instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Human - HeLa VDAC1 using Accell Human VDAC1 (7416) siRNA - Set of 4 from Dharmacon.

Manufacturer protocol

Download the product protocol from Dharmacon for Accell Human VDAC1 (7416) siRNA - Set of 4 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Human - HeLa VDAC1 using Accell Human VDAC1 (7416) siRNA - Set of 4 from Dharmacon. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.