ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature. Add mixture to cells and incubate for 20 minutes at room temperature. Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 24 hours.

Protocol tips
Mix the contents (dilute the siRNA and diluted DharmaFECT transfection reagent in serum-free medium) and incubate for 5 minutes at room temperature. Add mixture to cells and incubate for 20 minutes at room temperature. Replace culture medium with transfection medium and incubate cells at 37°C in 5% CO2 for 24 hours.

Publication protocol

For RNA interference assays, xCT ON-TARGET plus SMART pool siRNA (Dharmacon), or negative control siRNA, were delivered using the DharmaFECT transfection reagent according to the manufacturer’s instructions. To confirm initial transfection efficiency for siRNA experiments, PEL cells were transfected with green fluorescent protein (GFP)-tagged siRNA, and GFP expression determined by flow cytometry 24 h later as described previously [9]. Three independent transfections were performed for each experiment, and all samples were analyzed in triplicate for each transfection.

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Manufacturer protocol

Download the product protocol from Dharmacon for ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool below.

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