siRNA RUNX3

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells. The cells were incubated for 24 h and then serum starved for 48 h

Protocol tips
Vortex complex mixture (RNAiFect Transfection Reagent and diluted siRNA) for 10 seconds Incubate the samples for 10–15 min at room temperature (15–25°C) to allow formation of transfection complexes and add to cells. The cells were incubated for 24 h and then serum starved for 48 h

Publication protocol

RUNX3‐expressing SUIT‐2 and KLM‐1 cells were transfected with either scrambled negative control small interfering RNA (siRNA) or RUNX3 siRNA (Applied Biosystems, Foster City, CA) by using RNAiFect™ transfection reagent (Qiagen, Hilden, Germany). The cells were incubated for 24 h and then serum starved for 48 h. The MTT assay was performed as described in Section 2.8.

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Manufacturer protocol

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