siRNA FOXO1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C

Protocol tips
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C

Publication protocol

Cells at 60 to 80% confluence were co-transfected with miRNA inhibitors (miR-182 inhibitor, miR-183 inhibitor, both miR-182 and miR-183 inhibitors, or negative control miRNA inhibitor) along with Silencer select siRNA (FOXO1 (assay id #s5257 and #s5258) or negative control siRNA #1 (Thermo Fisher Scientific) using Lipofectamine RNAiMAX according to the manufacturer's protocols.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for siRNA FOXO1 below.

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