SignalSilence® SAPK/JNK siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentrations 25, 50 and 100 nM. Add 7.5 µl TransIT-X2 to the diluted mixture and incubate at room temperature for 15–30 minutes.  Add the TransIT-X2:sirna complexes to cells and incubate for 48 hours.

Protocol tips
siRNA concentrations 25, 50 and 100 nM. Add 7.5 µl TransIT-X2 to the diluted mixture and incubate at room temperature for 15–30 minutes.  Add the TransIT-X2:sirna complexes to cells and incubate for 48 hours.

Publication protocol

COV434 and KGN cells were seeded in 6-well plates and cultured until 70% confluency was obtained. TransIT-X2 Dynamic Delivery System was used to perform siRNA transfection at concentrations of 12.5, 25, 50 and 100 nM. The cells were incubated for 48 h post transfection, then harvested to determine the transfection efficiency by JNK expression on western blot.

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Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for SignalSilence® SAPK/JNK siRNA below.

Download manufacturer protocol

Videos

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