ApopTag® Fluorescein In Situ Apoptosis Detection Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Apply protinease K for 15 min Apply TdT enzyme and incubate in a humidified chamber at 37°C for 1 hour.

Protocol tips
Apply protinease K for 15 min Apply TdT enzyme and incubate in a humidified chamber at 37°C for 1 hour.

Publication protocol

The TUNEL assay was performed to detect apoptosis in the islets. The ApopTag Fluorescein in Situ Apoptosis Detection Kit (Chemicon International, Temecula, CA) was used according to the manufacturer's instructions. Fifteen samples were obtained by three independent experiments in each group. TUNEL+ cells and all cells were counted to calculate the ratio of DAPI+ cells

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Manufacturer protocol

Download the product protocol from Millipore for ApopTag® Fluorescein In Situ Apoptosis Detection Kit below.

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