DeadEnd™ Colorimetric TUNEL System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Fix with 4% paraformaldehyde solution or 10% buffered formalin in PBS for 15 minutes at room temperature. After rTdT reaction mix incubate the slides at 37°C for 60 minutes inside a humidified chamber

Protocol tips
Fix with 4% paraformaldehyde solution or 10% buffered formalin in PBS for 15 minutes at room temperature. After rTdT reaction mix incubate the slides at 37°C for 60 minutes inside a humidified chamber

Publication protocol

Islets were incubated with 3 μmol/L calcein-AM (Life Technologies) and 2.5 μmol/L propidium iodide (Sigma-Aldrich) before detection of absorbance/emission at 491/525 nm and 561/620 nm, respectively. The islet area occupied by dead cells was expressed as a unitary ratio versus that occupied by live cells. Apoptosis was assessed using a TUNEL staining kit (Promega) according to the manufacturer’s instructions, and islets were counterstained against insulin before detection of absorbance/emission at 491/525 nm and 561/620 nm, respectively.

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Papers

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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Colorimetric TUNEL System below.

Download manufacturer protocol

Videos

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