Accell Human VEGFC (7424) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 500,000 cells/ml	Final concentration -1 µM Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.  Incubate for 48 hours and plated in methylcellulose. Re-incubate for another 72 h.

Upstream tips
Seed 500,000 cells/ml
Protocol tips
Final concentration -1 µM Incubate TransIT-LT1 Reagent:siRNA complexe mixture at room temperature for 15–30 minutes to allow sufficient time for complexes to form and add to cells.  Incubate for 48 hours and plated in methylcellulose. Re-incubate for another 72 h.

Publication protocol

Accell SMARTpool siRNA pools for VEGFC, MYB, as well as a nontargeting pool, were obtained from GE Dharmacon and delivered in human BC cell lines according to the manufacturer’s recommendations. In brief, human BC cell lines were resuspended in Accell Delivery Media at a concentration of 500,000 cells/ml. siRNA pools were resuspended in 1× siRNA buffer and added to the cell suspension at a final concentration of 1 µM. Cells were incubated for 48 h and subsequently plated in methylcellulose. mRNA levels were assessed 72 h after treatment using primer pairs found in Table S6.

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Manufacturer protocol

Download the product protocol from Dharmacon for Accell Human VEGFC (7424) siRNA - SMARTpool below.

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