DeadEnd™ Colorimetric TUNEL System

TUNEL assay cell type - Mouse liver tissue

Experiment

TUNEL assay cell type - Mouse liver tissue

Product

DeadEnd™ Colorimetric TUNEL System from Promega

Manufacturer

Promega

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Tissue sections were deparaffinized in xylenes, gradually rehydrated in decreasing concentrations of ethanol,	- When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Protocol tips
- Tissue sections were deparaffinized in xylenes, gradually rehydrated in decreasing concentrations of ethanol,
Downstream tips
- When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Publication protocol

TUNEL-positive cells in liver sections were detected by commercial kit (Promega, G7130). Tissue sections were deparaffinized in xylenes, gradually rehydrated in decreasing concentrations of ethanol, and the assay performed according to the manufacturer's instructions. Under light microscopy, the numbers of TUNEL-positive cells in 10 randomly selected high power fields (400 × magnification) were counted per liver sample

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Discussion

4 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Mouse liver tissue using DeadEnd™ Colorimetric TUNEL System from Promega.

Paper title

Impaired macrophage autophagy increases the immune response in obese mice by promoting proinflammatory macrophage polarization

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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Colorimetric TUNEL System below.

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Videos

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