Protocol tips |
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Fix with fixation solution for 20 minutes at 15-25C. Add Tunel reaction mixture and incubate for 60min at 37C in a humidified chamber in the dark |
In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich has not yet been reviewed for this experiment
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Discussion
5 years ago
Author: Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
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Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.
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